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目的 探讨脐血CD3AK细胞诱导K5 6 2 细胞凋亡的作用。方法 用细胞形态学观察 ,DNA琼脂糖凝胶电泳 ,原位末端标记法检测 2 0例K5 6 2 细胞、2 3例CD3AK细胞诱导的K5 6 2细胞凋亡率。结果 K5 6 2 细胞自然凋亡率 (3.5 0± 0 .97) % ;经CD3AK细胞诱导的K5 6 2 细胞凋亡率 (2 7.38± 4.91) % ,二者之间具有显著性差异 (t=15 .1 P <0 .0 1)。结论 脐血CD3AK细胞能诱导K5 6 2 细胞凋亡。
Objective To investigate the effect of cord blood CD3AK cells on inducing apoptosis of K562 cells. Methods Cell morphology was observed by DNA agarose gel electrophoresis. The in situ end labeling method was used to detect the apoptosis rate of K562 cells induced by 20 K562 cells and 23 CD3AK cells. Results The natural apoptotic rate of K562 cells was (3.5 0 ± 0. 97)% and the apoptosis rate of K562 cells induced by CD3AK cells (2 7.38 ± 4.91)%. There was a significant difference between them (t = 15 .1 P <0 .0 1). Conclusion Cord blood CD3AK cells can induce apoptosis of K562 cells.