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目的:探讨脑室内注射脑源性神经营养因子(BDNF)抗体阻断内源性BDNF对大鼠海马-氧化氮合酶(NOS)阳性神经元的影响。方法:脑室内注射BDNF抗体一周后,采用Morris水迷宫进行行为检测;并用NADPH-黄递酶组化染色方法观察海马NOS阳性神经元数目的变化。结果:与对照组相比,实验组大鼠空间学习和记忆能力明显下降(P<0.01);实验组大鼠海马CA1区NOS阳性神经元数目(38.37±5.23)明显少于对照组(49.53±5.74)(P< 0.01);实验组DG区NOS阳性神经元数目(48.77±5.51)明显少于对照组(60.40±7.39)(P<0.01)。结论:脑室内注射BDNF抗体可导致大鼠空间学习记忆能力下降,海马NOS阳性神经元数目减少,提示BDNF对学习和记忆的影响可能与海马NOS阳性神经元数目的变化有关。
OBJECTIVE: To investigate the effect of intraventricular injection of BDNF on the hippocampal nitric oxide synthase (NOS) positive neurons in rats induced by endogenous BDNF. Methods: One week after intracerebroventricular injection of BDNF antibody, the Morris water maze was used for behavioral testing. The number of NOS positive neurons in the hippocampus was observed by NADPH-diaphorase histochemical staining. Results: Compared with the control group, the spatial learning and memory abilities in the experimental group were significantly decreased (P <0.01). The number of NOS positive neurons in the hippocampal CA1 subfield in the experimental group was significantly lower than that in the control group (38.37 ± 5.23) (49.53 ± 5.74) (P <0.01). The number of NOS positive neurons in DG area of experimental group (48.77 ± 5.51) was significantly less than that of control group (60.40 ± 7.39) (P <0.01). CONCLUSION: Intraventricular injection of BDNF antibody can decrease the spatial learning and memory capacity of rats and decrease the number of NOS positive neurons in hippocampus. It suggests that the effect of BDNF on learning and memory may be related to the changes of the number of NOS positive neurons in hippocampus.