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目的通过对阳性献血者的追踪检测,为建立献血者屏蔽和归队策略提供依据。方法对血筛HBs Ag、梅毒抗体ELISA及HBV NAT不合格献血者屏蔽8周后追踪采样,同时进行ELISA双试剂、NAT和相应补充、确证试验。结果共追踪到献血者61人。追踪检测结果:17名HBs Ag阳性献血者中,双试剂阳性8名:HBs Ag、NAT、抗-HBc和中和试验均阳性6人;HBs Ag、NAT和中和试验均阳性1人;HBs Ag和NAT阳性1人。单试剂阳性7名:HBs Ag和抗-HBc阳性2人;单独HBs Ag阳性5人。单试剂灰区2名:都是单独抗-HBc阳性。21名梅毒抗体阳性献血者中13人ELISA阳性,其中4人TPPA确证阳性,2人TPPA不确定;另外8人ELISA阴性。23名ELISA阴性NAT阳性献血者中,HBs Ag和NAT均阳性2人,单独NAT阳性6人,HBs Ag和NAT均阴性15人。结论针对不同情况的献血者应制定不同的屏蔽策略;同时可以通过对受屏蔽献血者的追踪检测,制定科学合理的归队策略。
Objective To track the detection of positive blood donors, to establish the basis for screening and rebuilding blood donors strategy. Methods Blood samples were screened for HBsAg, syphilis antibody ELISA and HBV NAT unqualified blood donors for 8 weeks, followed by ELISA double reagent, NAT and corresponding supplementation, confirmatory test. A total of 61 blood donors were traced. Tracing the test results: HBs Ag, NAT, anti-HBc and neutralization test were positive in 17 HBs Ag positive donors; HBsAg, NAT and neutralization test were positive in 1; HBs Ag and NAT positive 1 person. Seven single positive reagents: HBs Ag and anti-HBc positive 2; HBs Ag alone positive 5. Single reagent gray zone 2: all anti-HBc positive alone. Thirteen of 21 positive syphilis antibody-positive donors were ELISA positive, of whom 4 were positive for TPPA, 2 were undetermined for TPPA, and 8 were negative for ELISA. Among 23 ELISA negative NAT positive donors, 2 were positive for HBs Ag and NAT, 6 were positive for NAT alone, and 15 were negative for both HBs Ag and NAT. Conclusion Different donors should develop different masking strategies for different blood donors. At the same time, a scientific and reasonable resetting strategy should be established through the follow-up and testing of shielded blood donors.