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[Objective] The research aimed to isolate the genomic DNA in Morinda citrifolia Linn.and establish its ISSR-PCR reaction system.[Method] The Morinda citrifolia Linn.leaves of 3 germplasm resources from Hainan and 4 germplasm resources from America were chosen as test materials,the isolation of DNA in Morinda citrifolia Linn.and establishment of ISSR-PCR were studied.[Result] The results showed that DNA of Morinda citrifolia Linn.leaves with high quality were gained by the modified CTAB method.The isolated DNA could be used for ISSR analysis and 7 primers were found to amplify polymorphic products.[Conclusion]The rapid and efficient extraction method and the ISSR analysis procedure in this experiment have laid a good foundation for application of ISSR in Morinda citrifolia Linn.genetic research.
[Objective] The research aimed to isolate the genomic DNA in Morinda citrifolia Linn. And establish its ISSR-PCR reaction system. [Method] The Morinda citrifolia Linn.leaves of 3 germplasm resources from Hainan and 4 germplasm resources from America were chosen as test Materials, the isolation of DNA in Morinda citrifolia Linn. and establishment of ISSR-PCR were studied. [Result] The results showed that DNA of Morinda citrifolia Linn.leaves with high quality were gained by the modified CTAB method. used for ISSR analysis and 7 primers were amplified to polymorphic products. [Conclusion] The rapid and efficient extraction method and the ISSR analysis procedure in this experiment have laid a good foundation for application of ISSR in Morinda citrifolia Linn. genetic research.