目的了解武警某医院患者医院感染病原菌的构成及其耐药性变化情况,为临床抗菌药物使用提供依据。方法采用细菌分离鉴定技术与药敏试验方法,对武警部队某医院2012-2013年病人送检标本检测结果进行回顾性分析。结果该院医院感染主要革兰阳性菌(金黄色葡萄球菌)对哌拉西林/他唑巴坦及万古素敏感,对其它抗菌药物的耐药性均较高;主要革兰阴性杆菌均对喹诺酮类抗菌药物及三、四代头孢类抗菌药具有较高的耐药性;鲍曼不动杆菌与铜绿假单胞菌对常用抗生素的耐药性也较高。结论近两年该院主要条件致病菌的耐药情况较为严重,临床用药时应严格参照药敏结果,以降低细菌的耐药性。 Objective To understand the composition of hospital-acquired pathogens in a hospital in Armed Police Forces and their changes in drug resistance, so as to provide basis for the clinical use of antibacterial drugs. Methods The method of bacterial isolation and identification and drug susceptibility testing were used to analyze the results of the detection of patient samples from 2012 to 2013 in a hospital of Armed Police Forces. Results The hospital was infected with major Gram-positive bacteria (Staphylococcus aureus), which were sensitive to piperacillin / tazobactam and vancomycin. The resistance to other antibacterials was high. The main Gram-negative bacilli were resistant to quinolone Class antibacterial drugs and three or four generations of cephalosporins antibacterials have a higher resistance; Acinetobacter baumannii and Pseudomonas aeruginosa resistance to commonly used antibiotics are also higher. Conclusions The resistance of the pathogenic bacteria in the main condition of the hospital in the recent two years is more serious. In the clinical application, the drug susceptibility should be strictly referenced to reduce the drug resistance of the bacteria.