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目的探讨共刺激因子B7-H1和B7-H3在胆囊癌患者癌组织中的表达及临床意义。方法选取2006年8月至2010年8月间陕西省第二人民医院收治的40例胆囊癌患者,取手术切除的癌组织标本为观察组,取癌旁正常组织20例作为对照组,采用免疫组织化学SP法检测两组标本中B7-H1和B7-H3蛋白的表达水平。结果观察组标本中B7-H1和B7-H3蛋白的表达水平显著高于对照组标本。在观察组标本中表达定位主要浸润胞浆位置,染色呈淡黄色、棕黄色和棕褐色,观察组标本中B7-H1表达阳性细胞以高度浸润为主。胆囊癌(Ⅰ+Ⅱ)期中B7-H1蛋白表达阳性率高于胆囊癌(Ⅲ+Ⅳ)期,差异有统计学意义(χ~2=5.714,P=0.02),胆囊癌(Ⅰ+Ⅱ)期中B7-H3蛋白表达阳性率高于胆囊癌(Ⅲ+Ⅳ)期,差异有统计学意义(χ~2=4.224,P=0.04)。胆囊癌患者B7-H1和B7-H3的阳性表达组,其术后总生存率低于阴性表达组。结论 B7-H1和B7-H3的表达水平有助于胆囊癌的早期诊断,同时其含量可能是胆囊癌预后的独立预测因子之一。
Objective To investigate the expression and clinical significance of co-stimulatory factors B7-H1 and B7-H3 in gallbladder cancer. Methods Forty patients with gallbladder carcinoma admitted to the Second People’s Hospital of Shaanxi Province from August 2006 to August 2010 were selected as the observation group and 20 normal tissues adjacent to the tumor as control group. Histochemistry SP method was used to detect the expression of B7-H1 and B7-H3 protein in two groups of specimens. Results The expression of B7-H1 and B7-H3 in the observation group was significantly higher than that in the control group. In the observation group, the main location of the infiltrating cytoplasm was located and the staining was light yellow, brown and tan. The positive cells of B7-H1 expression in the observation group were mainly infiltrated. The positive rate of B7-H1 protein in gallbladder carcinoma (Ⅰ + Ⅱ) was higher than that in gallbladder carcinoma (Ⅲ + Ⅳ), the difference was statistically significant (χ2 = 5.714, P = 0.02) The positive rate of B7-H3 protein in stage was higher than that in gallbladder cancer (χ ~ = 2), the difference was statistically significant (χ ~ 2 = 4.224, P = 0.04). The positive expression rate of B7-H1 and B7-H3 in gallbladder cancer patients was lower than that in negative expression group. Conclusion The expression levels of B7-H1 and B7-H3 contribute to the early diagnosis of gallbladder cancer, and its content may be an independent prognostic factor for gallbladder cancer.