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目的 对１１株经输血传播病毒（ｔｒａｎｓｆｕｓｉｏｎ ｔｒａｎｓｍｉｔ ｖｉｒｕｓ，ＴＴＶ）进行基因型分析。方法 用巢式ＰＣＲ扩增不同地区不明病因的急、慢性肝炎，肝硬化、肝癌和自然健康人群ＴＴＶ ＤＮＡ片段，并对此巢式ＰＣＲ产物进行克隆，测序分析和同源性比较。结果 １１例标本的２２２ｂｐ（引物序列除外）的ＴＴＶ ＤＮＡ同源性比较结果为：武汉ＷＨ１、ＷＨ２、ＷＨ３ ３个患者血清中ＴＴＶ ＤＮＡ序列与Ｎ２２同源性分别为９７．０％、９７．０％和９８．０％；广州ＧＺ１、ＧＺ２、ＧＺ３ ３患者血清中ＴＴＶ ＤＮＡ序列与Ｎ２２同源性分别为９８．０％、９５．０％和９５．０％；山东ＳＤ２、ＳＤ３ ＴＴＶ ＤＮＡ序列与Ｎ２２同源性分别为９４、６％和９５．５％；广州ＧＺ４、山东ＳＤ１，新疆ＸＪ１患者ＴＴＶ ＤＮＡ序列与ＴＸ０１１同源性分别为９８．０％、９８．０％和９５．０％。结论 根据Ｏｋａｍｏｔｏ的分类方法，这１１株ＴＴＶ可属于两个亚型，即基因１型的ａ和ｂ亚型。
Objective To analyze genotypes of 11 strains of transfusion transmitted virus (TTV). Methods Nested PCR was used to amplify TTV DNA fragments of acute, chronic hepatitis, cirrhosis, hepatocellular carcinoma and natural healthy people of unknown etiology in different areas. The nested PCR products were cloned, sequenced and compared. Results The results of TTV DNA homology of 222bp (except for primer sequence) in 11 specimens were as follows: The homologies of TTV DNA sequences and N22 in serum of WH1, WH2 and WH3 in Wuhan were 97.0% and 97.0%, respectively % And 98.0%, respectively. The TTV DNA sequences and N22 homologies in GZ1, GZ2 and GZ3 3 patients in Guangzhou were 98.0%, 95.0% and 95.0%, respectively. The TTV DNA sequences of Shandong SD2 and SD3 N22 homology was 94.6% and 95.5%, respectively. The homologies of TTV DNA sequences to TX011 in Guangzhou GZ4, Shandong SD1 and Xinjiang XJ1 patients were 98.0%, 98.0% and 95.0%, respectively. Conclusion According to Okamoto’s classification method, these 11 strains of TTV may belong to two subtypes, namely the genotypes a and b subtypes.