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目的研究去甲斑蝥素(NCTD)、紫杉醇(PTX)及两者联合使用对前列腺癌细胞的抑制作用。方法实验分为3组:NCTD组、PTX组和联合组(NCTD+PTX)。NCTD组和PTX组:在处于指数增长的前列腺癌细胞株DU145中,加入不同浓度(12.5,25.0,50.0,100.0,200.0,400.0,800.0μmol·L~(-1))的NCTD和PTX;联合组:NCTD与PTX按1∶1浓度配比,重复第1组操作。48 h后再加入3-(4,5-二甲基噻唑-2)-2,5-二苯基四氮唑溴盐(MTT)工作液20μL,培养4 h后吸出上层液体,加入DMSO 200μL进行溶解。以离心培养法测定DU145的复苏率和计算细胞生长抑制率;以MTT法检测半数抑制浓度。以模拟联合指数考察两药物是否具有协同作用。结果上述由小到大7个浓度NCTD组对癌细胞的抑制率分别为0.12%,0.14%,0.25%,0.30%,0.58%,0.70%,0.74%,PTX组分别为0.42%,0.43%,0.44%,0.46%,0.46%,0.47%,0.52%,联合组分别为0.53%,0.55%,0.56%,0.61%,0.66%,0.75%,0.79%,联合组对癌细胞的抑制率高于NCTD组与PTX组,差异均有统计学意义(均P<0.05)。随着两药剂量的增加,模拟联合指数值越来越高,且小于1,最低为0.07±0.01,最高为0.59±0.20,与最低浓度比较差异有统计学意义(P<0.05),表明NCTD、PTX联用时,两者间有协同作用,且随着剂量的增加,协同作用更明显。结论 NCTD与PTX对非依赖前列腺癌细胞均有抑制效果,且浓度越高抑制作用越强;两者联合使用作用更强。
Objective To study the inhibitory effect of norcantharidin (NCTD), paclitaxel (PTX) and their combination on prostate cancer cells. Methods The experiment was divided into 3 groups: NCTD group, PTX group and combined group (NCTD + PTX). NCTD group and PTX group: NCTD and PTX at different concentrations (12.5,25.0,50.0,100.0,200.0,400.0,800.0μmol·L -1) were added into the exponential growth of prostate cancer cell line DU145; Group: NCTD and PTX 1: 1 concentration ratio, repeat the first group of operations. After 48 h, 20 μL of working solution of 3- (4,5-dimethylthiazol-2-yl) -2,5-diphenyltetrazolium bromide (MTT) was added. After 4 h of incubation, the supernatant fluid was aspirated and 200 μL of DMSO Dissolve. The recovery rate of DU145 and the cell growth inhibition rate were determined by centrifugation culture method. The half inhibitory concentration was detected by MTT assay. To simulate the joint index to investigate whether the two drugs have a synergistic effect. Results The inhibitory rates of NCTD treated with NCTD at concentrations ranging from 7 to 10 were 0.12%, 0.14%, 0.25%, 0.30%, 0.58%, 0.70% and 0.74%, respectively, while those in PTX group were 0.42% and 0.43% 0.44%, 0.46%, 0.46%, 0.47% and 0.52% in the combined group were 0.53%, 0.55%, 0.56%, 0.61%, 0.66%, 0.75% and 0.79% NCTD group and PTX group, the difference was statistically significant (P <0.05). With the increase of the dose of the two drugs, the simulated combined index value was higher and higher, and less than 1, the lowest was 0.07 ± 0.01, the highest was 0.59 ± 0.20, with the lowest concentration was statistically significant difference (P <0.05), NCTD , PTX combination, the synergy between the two, and with the increase of dose, the synergistic effect is more obvious. Conclusions Both NCTD and PTX have inhibitory effects on non-prostate cancer cells, and the higher the concentration is, the stronger the inhibition is. The combination of NCTD and PTX is more effective.