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矮秆和半矮秆基因的利用及作用机制一直是作物育种和分子遗传研究的重要内容.sd-t（t）是从籼稻矮秆材料矮泰引-2中发现的一个与sd-1不等位的新半矮秆基因.利用由矮泰引-2与无叶舌标志基因系B30杂交产生的包含474个单株的F2群体,将sd-t（t）基因定位在水稻第4染色体上RFLP标记R1408B和R514之间,距R514和R1408B的遗传距离分别为1.1和4.5cM.利用水稻品系IRBB56的BAC文库构建了sd-t（t）位点的跨叠克隆群,并确定R514与R1408B之间的物理距离大约为147kb,为进一步克隆sd-t(t)基因奠定了基础.
The utilization and mechanism of dwarf and semi-dwarf genes has been an important part of crop breeding and molecular genetic studies.sd-1 (t) The new semi-dwarfing gene was used to map the sd-t (t) gene in F2 population of rice (Oryza sativa L.) The genetic distances between RFLP markers R1408B and R514 were 1.1 and 4.5 cM, respectively, from R514 and R1408B.The transposable clonal population of sd-t (t) was constructed using the BAC library of rice line IRBB56, The physical distance between R1408B was about 147kb, which laid the foundation for further cloning sd-t (t) gene.