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目的 探讨乙肝病毒 (HBV) X基因与肝癌发病的关系 ,为从分子水平上研究肝癌病因提供有关信息。方法 采用 PCR技术对 HBV不同状态的 1 0 2例原发性肝细胞癌 (PHC)、36例肝硬化 (L C)及 2 8例慢性肝炎 (CH)血清中的 HBVX基因进行了检测。结果 在 HBV5项标志均阴性的 53例 PHC检出 X基因 2 0例 (37.7% ) ,31例 LC中检出 5例 (1 6.1 % ) ,1 1例 CH中检出 2例 (1 8.2 % )。所有HBV X基因阳性血清 HBV S基因扩增产物检测结果 ,31例 PHC中检出 S基因阴性 2 0例 (64.5% ) ,8例 LC中检出 5例 (62 .5% ) ,1 1例 CH中全部为阳性。而在肝血管瘤和肝囊肿等其他肝病中未发现 X基因 (0 /35)。结论 HBV X基因整合于肝细胞染色体后与肝硬化、肝癌的发生发展关系密切 ;整合的 HBV X基因与p53抑癌基因的失活密切相关 ,HBV X基因的整合可能是肝癌发生的一个重要原因。
Objective To investigate the relationship between the hepatitis B virus (HBV) X gene and the onset of liver cancer, and to provide relevant information for the study of the cause of liver cancer at the molecular level. Methods PCR was used to detect the HBVX gene in serum of 102 patients with different status of HBV, primary hepatocellular carcinoma (PHC), 36 cases of cirrhosis (L C) and 28 cases of chronic hepatitis (CH). RESULTS: Twenty cases (37.7%) of X genes were detected in 53 PHCs with negative HBV5 markers, 5 (16.1%) were detected in 31 LCs, and 2 (18.2%) were detected in 11 cases of CH. ). All HBV X gene positive serum HBV S gene amplification products were detected in 31 cases of PHC negative S gene detected in 20 cases (64.5%), 8 cases of LC detected in 5 cases (62.5%), 11 cases All the CHs are positive. However, no X gene (0/35) was found in liver diseases such as hemangiomas and hepatic cysts. Conclusion The integration of HBV X gene into chromosomes of hepatocytes is closely related to the development of liver cirrhosis and liver cancer. The integration of HBV X gene is closely related to the inactivation of p53 tumor suppressor genes. Integration of HBV X gene may be an important cause of liver cancer. .