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本文制备了含金纳米粒子的聚电解质微囊,并进行了表征。以碳酸钙粒子为模板,在其表面组装聚烯丙基胺盐酸盐[poly(allyamine hydrochloride),PAH]和金纳米粒子,得到以碳酸钙粒子为母核,PAH/金纳米粒子为壳的核壳结构微粒。用乙二胺四乙酸二钠(EDTA)溶解碳酸钙,即可得到含有金纳米粒子的聚电解质微囊。用扫描电镜表征碳酸钙粒子、含金纳米粒子的聚电解质微囊去掉母核前后的形状,可观察到碳酸钙粒子表面包裹金纳米粒子前后的差别。用显微镜表征了微囊在溶液中的形态,微囊在水中分散性良好。载入异硫氰酸荧光素标记的牛血清白蛋白(FITC-bovine serum albumin,FITC-BSA)作为模型药物,用荧光显微镜可以观察到微囊内有一定的荧光强度,检测得到牛血清白蛋白的包封率为(34.31±2.44)%,聚电解质微囊载药量为(43.75±3.12)mg·g-1。
In this paper, polyelectrolyte microcapsules containing gold nanoparticles were prepared and characterized. Using calcium carbonate particles as a template, poly (allyamine hydrochloride) (PAH) and gold nanoparticles were assembled on the surface to obtain calcium carbonate particles as a mother nucleus and PAH / gold nanoparticles as a shell Core-shell structure particles. Dissolution of calcium carbonate with disodium ethylenediaminetetraacetate (EDTA) yields polyelectrolyte microcapsules containing gold nanoparticles. Characterization of calcium carbonate particles by scanning electron microscopy, gold nanoparticles containing polyelectrolyte microcapsules before and after removal of the shape of the mother nuclei can be observed on the surface of calcium carbonate particles coated gold nanoparticles before and after the difference. The morphology of the microcapsules in solution was characterized by a microscope and the microcapsules were well dispersed in water. FITC-BSA (FITC-BSA) was loaded as a model drug, and fluorescence intensity was detected in the microcapsules by fluorescence microscopy. The concentration of bovine serum albumin (34.31 ± 2.44)% and polyelectrolyte microcapsules (43.75 ± 3.12) mg · g-1, respectively.