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在做初高中的几个生理实验时,根据班次需要,在讲细胞分裂前1—2天,把蚕豆发芽。具体做法是,把锯末用温水和湿,温度以手捏成团而水不下滴为宜。将和好的锯末放于容器中,厚约2寸左右,将种子均匀地埋在其中,在温暖的地方4—6天后,胚根可长出1—2厘米。此时根尖生长点细胞正在进行旺盛的有丝分裂,且蚕豆细胞中染色体数目少(6对,12条)染色体外形大,所以便于染色体的观察和计数。此时恰好可做高中生物“观察植物细胞有丝分裂”实验。注意因胞间层不如洋葱细胞紧密,故离析时间应比洋葱根尖少3—5分钟。把剪去主根的蚕豆埋入锯末中,适当补水,继续培
In doing a few high school physiological experiments, according to the needs of the class, about 1-2 days before cell division, the germination of broad beans. The specific approach is to use sawdust with warm water and wet, the temperature to hand into the group and no drop of water is appropriate. The good sawdust in the container, about 2 inches thick, the seeds buried in them evenly, 4-6 days in warm places, the radicle can grow 1-2 cm. At this point apical growing point cells are exuberant mitosis, and the number of chromosomes in Vicia faba few (6 pairs, 12) chromosome shape, so easy to observe and count chromosomes. At this time just do high school biology “observation plant cell mitosis” experiment. Note that due to the intercellular layer of onion cells close, so the isolation time should be 3-5 minutes less than onion root tips. Cut the main root of broad beans buried in sawdust, appropriate replenishment, continue to cultivate