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用紫外 -可见 ( UV-Vis)吸收光谱和电化学方法研究 Eu3+ 与微过氧化物酶 -8( MP-8)相互作用的机理 ,发现 Eu3+优先与 MP-8中血红素基团的 2个丙酸基的羧基氧发生强的配位作用 ,导致 MP-8分子中血红素基团的非平面性、暴露程度和电化学可逆性的增加 .过剩的 Eu3+与 MP-8分子中肽链上的含氧基团发生弱的相互作用 ,对血红素基团结构的影响较小
The mechanism of the interaction between Eu3 + and microperoxidase-8 (MP-8) was studied by UV-Vis absorption spectroscopy and electrochemical methods. It was found that Eu3 + preferentially interacts with two of the heme groups in MP-8 The strong coordination of the carboxyl groups of the propionate group leads to the increase of the non-planarity, the degree of exposure and the electrochemical reversibility of the heme group in the MP-8 molecule. The excess Eu3 + and the peptide chain in the MP-8 molecule Of the oxygen-containing groups weak interaction, the structure of the heme group less