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大肠杆菌表达的戊型肝炎病毒 (HEV)衣壳蛋白ORF2片段HEV 2 39重组蛋白颗粒 ,经铝佐剂吸附后 ,分别以5 μg、1 0 μg和 2 0 μg剂量免疫恒河猴 ,2 8天时以相同剂量加强免疫 1次 ,3周后分别以不同病毒滴度的基因Ⅰ型或基因Ⅳ型HEV静脉攻击。结果 ,加强后 3周 ,3个免疫剂量组猴的抗体几何平均滴度分别为 1∶2 71 75、1∶344 0 9、1∶4 16 0 7,以世界卫生组织参比血清定量 ,则分别为 1 0 98IU/ml,1 35 7IU/ml、1 72 4IU/ml。每个剂量免疫组及对照组各有 3只猴接受 1 0 7病毒滴度基因Ⅰ型HEV感染 ,对照组 3只猴均被成功感染 ,2只出现肝炎 ;2 0 μg免疫组 3只猴均未被感染 ;1 0 μg和 5 μg免疫组各有 2只猴未被感染 ,另 1只猴出现短暂感染 ,免疫猴均未出现肝炎。 3个剂量免疫组及对照组另外 3只猴 ,接受 1 0 4病毒滴度基因Ⅰ型HEV感染 ,对照组 3只猴均被感染 ,1只出现肝炎 ;而免疫猴均未被感染 ,也未出现肝炎。 3只 1 0 μg免疫猴和 3只对照猴分别接受 1 0 7病毒滴度基因Ⅳ型HEV感染 ,对照组均被感染并出现肝炎 ,而免疫组均未出现肝炎 ,有 2只未被感染 ,另 1只被短暂感染。另有 3只 1 0 μg免疫猴和 3只对照猴分别接受 1 0 4病毒滴度基因Ⅳ型HEV感染 ,对照组均被感染 ,而免疫组均未被感染。这些结果表明 :HEV
Escherichia coli HEV capsid protein ORF2 fragment HEV 2 39 recombinant protein particles, after adsorption by aluminum adjuvant, were immunized with 5 μg, 10 μg and 20 μg doses of rhesus monkey, 28 Day to strengthen the same dose of 1 times, 3 weeks after the different virus titer gene type I or type IV HEV vein attack. As a result, the geometric mean titers of monkeys immunized with the three immunized doses were 1: 2 71 75, 1:34 4 0 9, 1: 4 16 0 7, respectively, three weeks after the booster, and were quantified with the WHO reference serum Respectively, 1 98 IU / ml, 1 35 7 IU / ml, 1 72 4 IU / ml. Three monkeys in each dose immunized group and control group were infected with the type I HEV of 1 0 7 virus titer, 3 monkeys in the control group were successfully infected, and 2 were hepatitis. In the 20 μg immunized group, 3 monkeys Were not infected. Two monkeys in the 10 μg and 5 μg immunized groups were not infected, while the other one had a transient infection. All immunized monkeys did not show hepatitis. Three other immunized groups and three control monkeys received infection with genotype I HEV of 104 virus titers. Three monkeys in the control group were infected and one developed hepatitis. None of the immunized monkeys were infected or not Hepatitis appears. Three 1 0 μg immunized monkeys and 3 control monkeys were infected with a virus titer of type IV HEV of 1 0 7 respectively. All the control groups were infected with hepatitis, but no hepatitis was found in the immunized group and 2 were not infected. The other one was briefly infected. Three other 10 μg immunized monkeys and three control monkeys were infected with the virus of type IV HEV of 104 virus titer respectively. All the control groups were infected, but none of the immunized groups were infected. These results show that: HEV