论文部分内容阅读
背景:随着基因工程以及肿瘤生物分子学等新兴学科的发展壮大,基因治疗肿瘤成为一种新的治疗模式。目的:探讨KDR基因沉默对乳腺癌MCF-7细胞增殖能力和侵袭能力的影响。方法:设计针对KDR基因的小分子干扰RNA(siR NA)序列,转染人乳腺癌MCF-7细胞,应用RT-PCR及Western blot法检测沉默KDR基因后MCF-7细胞KDR mR NA及蛋白的表达;应用流式细胞仪、CCK-8增殖实验、小室侵袭实验检测沉默KDR基因后乳腺癌MCF-7细胞的细胞周期、增殖能力、侵袭能力的变化。结果与结论:KDR基因沉默48 h后,乳腺癌MCF-7细胞KDR mR NA及蛋白表达明显降低;乳腺癌MCF-7细胞停滞在G0/G1周期,S期的细胞数目减低,细胞增殖得到显著抑制,穿过滤膜的细胞数量明显减少。上述结果表明沉默KDR基因后乳腺癌MCF-7细胞的增殖、侵袭能力得到明显抑制,说明KDR基因沉默可能成为新的有效治疗乳腺癌的靶点。
Background: With the development of new disciplines such as genetic engineering and tumor biomolecules, gene therapy has become a new treatment model. Objective: To investigate the effect of KDR gene silencing on the proliferation and invasiveness of breast cancer MCF-7 cells. Methods: Human breast cancer cell line MCF-7 was transfected with the small interfering RNA (siR NA) against KDR gene. The KDR mRNA and protein of MCF-7 cells were detected by RT-PCR and Western blot. The cell cycle, proliferation and invasion of breast cancer MCF-7 cells were detected by flow cytometry, CCK-8 proliferation assay and cell invasion assay. RESULTS AND CONCLUSION: After KDR gene silencing for 48 h, the KDR mR NA and protein expression of breast cancer MCF-7 cells were significantly reduced. The number of MCF-7 cells arrested in G0 / G1 cycle and S phase decreased significantly Inhibition, the number of cells through the filter significantly reduced. The above results show that the proliferation and invasion ability of MCF-7 breast cancer cells after silencing KDR gene are significantly inhibited, indicating that KDR gene silencing may be a new target for effective treatment of breast cancer.