论文部分内容阅读
我们将苏云金芽孢杆菌(Bacillus thuringiensis)的两个变种 B.t.kurstaki HD-1(cryIA(b)和 HD-73(cryIA(c)的抗虫基因进行修饰后转化棉花并使其得到表达,其表达的抗虫蛋白能有效地毒杀农业生产上重要的鳞翅目害虫。在实验室中测定了这些转基因棉花叶组织的总抗虫能力,试验所用的两个鳞翅目昆虫是对 B.t.k.抗虫蛋白相对敏感的甘蓝尺蠖(Trichoplusia ni,cabbage looper)和敏感性比甘蓝尺蠖低100倍的甜菜粘虫(Spodoptera exigua,beet armyworm)。在高虫口密度胁迫下用棉铃虫(Heliothis zea.cotton bollworm)对整株棉花进行抗虫试验,结果显示出蕾、铃的有效抗虫性。对棉花植株进行的免疫(?)迹分析表明,抗虫蛋白的含量为总可溶性蛋白的0.05～0.1%。我们将这些结果视为向农业生产上推广这种经过抗虫遗传改良的经济价值高、种植面广的农作物的主要一步。
We transformed cotton and expressed it by modifying the Btkurstaki HD-1 (cryIA (b) and HD-73 (cryIA (c)) insect genes of Bacillus thuringiensis, Insect-resistant proteins are effective in killing lepidopteran pests that are important in agricultural production.The total insect resistance of these transgenic cotton leaf tissues was measured in the laboratory and the two Lepidoptera insects used in the experiment were resistant to the Btk insect-resistant protein The relatively sensitive Trichoplusia ni cabbage looper and the susceptible Spodoptera exigua (beet armyworm) were 100 times lower than the cabbage loosestrifera.The Heliothis zea.cotton bollworm The whole cotton was tested for its resistance to insects, and the results showed that the effective insect resistance of buds and bolls.The analysis of the immune (?) Trace of the cotton plants showed that the content of the insecticidal protein was 0.05-0.1% of the total soluble protein. These results are viewed as a major step forward in agricultural production to promote this economically advanced and well-planted crop that has been genetically modified by insect resistance.