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目的了解2012-2016年天津地区住院腹泻儿童的病毒感染情况和流行规律。方法收集天津市儿童医院2012年1月-2016年12月住院的腹泻儿童粪便标本,采用胶体金免疫层析法快速检测轮状病毒(RV)和腺病毒(AdV),采用酶联免疫吸附试验方法(ELISA)进行星状病毒(AstV)抗原检测。结果 11 746份粪便标本中,阳性例数为1 430份,总阳性检出率为12.17%,三种病毒中RV阳性检出率最高(9.46%),其次为AdV(1.83%)、AstV(0.97%);男女阳性检出率差异无统计学意义(χ~2=0.279,P=0.598);各月龄组均能检测到腹泻病毒,其中12~23月龄组的阳性检出率最高(20.02%);RV在12月份阳性检出率最高,其次是11月份,8月份最低;AstV在1月份阳性检出率最高,全年低流行散发;AdV在6月份阳性检出率最高,5~8月呈现一个小高峰。结论 RV是2012-2016年天津地区住院腹泻患儿感染的主要病原之一,秋冬季节为发病高峰。AstV和AdV的感染在婴幼儿腹泻中不容忽视。同时存在一定比例的混合感染。
Objective To understand the prevalence and prevalence of viral infection in hospitalized children with diarrhea in Tianjin from 2012 to 2016. Methods Stool specimens from children hospitalized in Tianjin Children’s Hospital from January 2012 to December 2016 were collected. The rotavirus (RV) and adenovirus (AdV) were detected by colloidal gold immunochromatography. The enzyme linked immunosorbent assay Methods (ELISA) for detection of Astrovirus (AstV) antigen. Results Among 11 746 stool samples, the number of positive samples was 1 430 and the total positive detection rate was 12.17%. The positive rate of RV in all three viruses was the highest (9.46%), followed by AdV (1.83%), AstV ( 0.97%). No significant difference was found in the positive rate of male and female (χ ~ 2 = 0.279, P = 0.598). The diarrhea virus was detected in all age groups, of which the positive rate was 12 to 23 months (20.02%). The positive rate of RV was the highest in December, followed by November and the lowest in August. The positive rate of AstV was the highest in January and low in the whole year. The positive rate of AdV in June was the highest, 5 to August showed a small peak. Conclusion RV is one of the major pathogens in children with in-hospital diarrhea in Tianjin in 2012-2016, with peak incidence in autumn and winter. AstV and AdV infection in infants and young children can not be ignored diarrhea. At the same time there is a certain percentage of mixed infections.